BMPR2 Editing in Fibroblast NIH3T3 using CRISPR/Cas9 Affecting BMPR2 mRNA Expression and Proliferation
نویسندگان
چکیده
BACKGROUND: Bone Morphogenetic Protein Receptor II (BMPR2) deficiency is associated with the pathologic development of pulmonary vascular changes in Pulmonary Arterial Hypertension (PAH). Fibroblast most abundant cell vascular. However, there only a little information regarding effect BMPR2 fibroblast. This study aims to understand fibroblasts.METHODS: applied CRISPR/Cas9 technique edit BMPPR2 NIH-3T3 cells. The transfection for editing into cells was done by using chitosan nanoparticles. evaluation and Transforming Growth Factor (TGF)-? mRNA expression Quantitative real-time polymerase chain reaction. assessment edited proliferation scratch test assay.RESULTS: CRISPR/Cas9-edited group lower than untreated group. higher TGF-? groups similar.CONCLUSION: fibroblast increase ability proliferate.KEYWORDS: BMPR2, PAH, NIH-3T3, CRISPR/Cas9,
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ژورنال
عنوان ژورنال: The Indonesian Biomedical Journal
سال: 2022
ISSN: ['2355-9179', '2085-3297']
DOI: https://doi.org/10.18585/inabj.v14i1.1724